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Image Search Results
Journal: The Journal of Biological Chemistry
Article Title: Regulation of Pyruvate Dehydrogenase Kinase 4 (PDK4) by Thyroid Hormone
doi: 10.1074/jbc.M109.039081
Figure Lengend Snippet: Effect of PGC-1α knockdown on various T3-responsive genes. Rat primary hepatocytes were infected with adenoviral vectors expressing shRNA for PGC-1α (Ad-siPGC-1α) or expressing control-shRNA (Ad-NC). Hepatocytes were treated with T3 at a concentration of 100 nm for 24 h. The mRNA abundance of the indicated genes was measured by real time PCR. The infections were repeated three to four times on independent plates of cells. A, fold induction of the PGC-1α gene expression in hepatocytes in comparison with hepatocytes infected with Ad-NC and nontreated with T3. B, rPDK4; C, CPT-1a; D, PEPCK; E, fatty-acid synthase (FASN); and F, TRβ mRNA abundance was also measured. The data are expressed as the mean of the fold induction ± S.E. of mRNA abundance relative to cells nontreated with T3 (*, p value 0.01 to 0.05; **, p value 0.001 to 0.01).
Article Snippet:
Techniques: Knockdown, Infection, Expressing, shRNA, Control, Concentration Assay, Real-time Polymerase Chain Reaction, Gene Expression, Comparison